Alpha-Keratin: Ultrafine Structure of α-Keratin

Macromolecular structure and organization of alpha keratin.

Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19

Introduction To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. Methods: Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea wer...

Structure and composition of soluble feather keratin.

Blackburn, S. & Lowther, A. G. (1951). Biochem. J. 48, 126. Bowes, J. H. & Moss, J. A. (1953). Biochem. J. 55, 735. Courts, A. (1954). Biochem. J. 58, 70. Craig, L. C. & Craig, D. (1950). In Technique of Organic Chemistry, vol. 3, chap. 4. Ed. by Weissberger, A. New York: Interscience Publishers, Inc. Craig, L. C. & Post, 0. (1949). Analyt. Chem. 21, 500. Felix, K. (1953). In The Chemical Struc...

Dynamics of keratin assembly: exogenous type I keratin rapidly associates with type II keratin in vivo

Keratin intermediate filaments (IF) are obligate heteropolymers containing equal amounts of type I and type II keratin. We have previously shown that microinjected biotinylated type I keratin is rapidly incorporated into endogenous bundles of keratin IF (tonofilaments) of PtK2 cells. In this study we show that the earliest steps in the assembly of keratin subunits into tonofilaments involve the...

Determination of keratin degradation by fungi using keratin azure.

Azure dye-impregnated sheep's wool keratin (keratin azure) was incorporated in a high pH medium and overlaid on a keratin-free basal medium. The release and diffusion of the azure dye into the lower layer indicated production of keratinase. Fifty-eight fungal taxa, including 49 members of the Arthrodermataceae, Gymnoascaceae and Onygenaceae (Order Onygenales), were assessed for keratin degradat...